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Rheumatoid arthritis: RA synovium harbours distinct fibroblast subsets
文章来源:临床药理研究所    发布时间:2018-04-20    浏览次数:276

Synovial fibroblasts are considered to actively contribute to the disease process in rheumatoid arthritis (RA) through various functions. A new study published inNature Communicationsidentifies several distinct subsets of these fibroblasts, including a subset of CD34THY1+cells that is expanded in RA and could carry out particular pathogenic functions.

The researchers first examined the protein expression of a range of surface markers on fibroblasts isolated from synovial tissue collected from patients with RA or osteoarthritis (OA) undergoing joint replacement surgery or from patients with RA undergoing synovectomy. Two main populations of fibroblast were identified on the basis of CD34 expression; CD34+and CD34fibroblasts were then further subdivided into seven phenotypic populations according to expression of THY1 (also known as CD90) and cadherin 11 (CDH11). These seven gated populations also had distinct gene expression profiles in both microarray and RNA-seq data sets. The cells were grouped into three major subsets — CD34THY1, CD34THY1+and CD34+fibroblasts — using principal component analysis (PCA) of the microarray data; interestingly, unbiased clustering of fibroblasts by single-cell RNA-seq confirmed these three subsets.

The proportions of the fibroblast subsets differed between synovial tissue from patients with RA and that from patients with OA, with CD34THY1+cells in particular being threefold more abundant in RA than in OA (22% versus 8%). In biopsy-obtained synovial tissue from the knees of patients with RA, the proportion of CD34THY1+fibroblasts correlated with the proportion of infiltrating leukocytes by flow cytometry, as well as with histological synovitis and synovial hypertrophy as assessed by use of ultrasonography. These findings suggest that the altered proportions of fibroblast subsets in RA reflect tissue inflammation. No correlation was found between the proportion of CD34THY1+fibroblasts and disease duration (as measured in years).

In RA tissue, CD34THY1+fibroblasts localized around blood vessels in the sublining area of the synovium. Notably, most of the CD34THY1+fibroblast population in RA tissue were also positive for CDH11, the expression of which is characteristic of fibroblasts and has been linked to the pathological activity of these cells. Moreover, the transcriptomic profile of CD34THY1+fibroblasts correlated with cellular functions in vitro, including invasion and migratory behaviour, secretion of pro-inflammatory cytokines and proliferation.

Together, the findings suggest that distinct fibroblast subsets perform different molecular functions in RA. The researchers suggest that the identification of pathogenic fibroblast subsets could reveal new therapeutic targets.

Nature Reviews Rheumatology14,250(2018)